DNA replication

DNA Replication :
-existing DNA strands to serve as templates for new complementary strands
- Semiconservative, new DNA has 1 old 1 new strand

1, Helicase untwist and separate template DNA strands from the origin of replication, break hydrogen bonds and moves along the direction of leading strand
2, SS binding proteins keep the unpaired template strands apart during replication
3, Gyrase releases any tension brought about by the unwinding of the DNA strands
4, Primase attaches RNA primer as soon as there is a single strand
5, DNA polymerase 3 catalyze the elongation of new DNA and attaches free flowing nucleosides on to the single strand
6, Leading strand is replicated continuously towards replication fork while lagging strand is replicated in okazaki fragments. The growing direction of the new DNA strand is always 5 to 3. Therefore leading strand   always starts with 3 prime.
7, DNA polymerase 1 checks over the new replicated DNA strand and remove the RNA primer.
8, DNA ligase adds sugar phosphate backbone between the Okazaki fragments ( fill in gaps)

list of Enzymes
DNA polymerase 3
DNA polymerase 1
DNA ligase
helicase
gyrase
primase

Replication fork is where the parental DNA strands hasn't untwist. Replication bubbles allow DNA replication to speed up therefore the untwisted DNA would not be attacked by enzymes while replicating.